Sequenciamento Shotgun: diferenças entre revisões
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Neste exemplo extremamente simplificado, nenhuma das leituras cobrem o comprimento total da sequência original, mas as quatro leituras podem ser montadas na sequência original utilizando a sobreposição das suas extremidades para as alinhar e ordenar. Na realidade, esse processo usa quantidades enormes de informação que são repletas de ambiguidades e erros de seqüenciamento.
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Many overlapping reads for each segment of the original DNA are necessary to overcome these difficulties and accurately assemble the sequence. For example, to complete the [[Human Genome Project]], most of the human genome was sequenced at 12X or greater ''coverage''; that is, each base in the final sequence was present on average in 12 different reads. Even so, current methods have failed to isolate or assemble reliable sequence for approximately 1% of the ([[Euchromatin|euchromatic]]) human genome, as of 2004.<ref name=HGS2004>{{cite journal|last1=Human Genome Sequencing Consortium|first1=International|title=Finishing the euchromatic sequence of the human genome|journal=Nature|date=21 October 2004|volume=431|issue=7011|pages=931–945|doi=10.1038/nature03001}}</ref>
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