Sequenciamento Shotgun: diferenças entre revisões

O [[Método de Sanger|método de terminação de cadeia]] de [[sequenciamento de DNA]] (ou "[Método de Sanger]]" por ter sido desenvolvido por Frederick Sanger]]) só pode ser usado para cadeias muito curtas de 100 a 1000 [[par de base|pares de bases]]. <!-- Longer sequences are subdivided into smaller fragments that can be sequenced separately, and subsequently they are re-assembled to give the overall sequence. Two principal methods are used for this: [[primer walking]] (or "chromosome walking") which progresses through the entire strand piece by piece, and shotgun sequencing which is a faster but more complex process that uses random fragments.