Wikipédia

Ku70

gene da espécie Homo sapiens

Ku70 é uma proteína que em humanos é codificada pelo gene XRCC6.[1]Ku70 junto com Ku80, conformam um heterodimero denominado proteína Ku, que se liga as extremidades da dupla fita de DNA que foi cortado, e é requerida para o processo de recombinação não homóloga durante a reparação do DNA. Também é necessária para o processo de recombinação V(D)J, que consegue aumentar a diversidade antigênica do sistema imune de mamíferos utilizando o processo de recombinação não homóloga. Ku também é necessária para manter o tamanho do telômero e silenciamento de genes subteloméricos.

Estrutura tridimensional da Ku70

Ku foi originalmente identificado em pacientes com lúpus eritematoso sistêmico , no qual, após ser submetido a análise, eles encontraram altos níveis de autoanticorpos contra essa proteína.[1]

Outros nomes editar

  • Lupus Ku autoantigen protein p70
  • ATP-dependent DNA helicase 2 subunit 1
  • X-ray repair complementing defective repair in Chinese hamster cells 6
  • X-ray repair cross-complementing 6 (XRCC6)

Interações editar

Interage com GCN5L2,[2] PTTG1,[3] HOXC4,[4] CHEK1,[5] Werner syndrome ATP-dependent helicase,[6][7] RPA2,[8] CBX5,[9] CREB-binding protein,[2] Neutrophil cytosolic factor 4,[10] MRE11A,[11] NCOA6,[12][13] Ku80,[2][14][15][16][17] TERF2,[17] VAV1,[18] Telomerase reverse transcriptase[19] e PCNA.[20][21]

Referências

  1. ↑ a b «Entrez Gene: XRCC6 X-ray repair complementing defective repair in Chinese hamster cells 6 (Ku autoantigen, 70kDa)» 
  2. ↑ a b c Barlev, N A; Poltoratsky V, Owen-Hughes T, Ying C, Liu L, Workman J L, Berger S L (1998). «Repression of GCN5 histone acetyltransferase activity via bromodomain-mediated binding and phosphorylation by the Ku-DNA-dependent protein kinase complex». UNITED STATES. Mol. Cell. Biol. 18 (3): 1349–58. ISSN 0270-7306. PMC 108848 . PMID 9488450 
  3. ↑ Romero, F; Multon M C, Ramos-Morales F, Domínguez A, Bernal J A, Pintor-Toro J A, Tortolero M (2001). «Human securin, hPTTG, is associated with Ku heterodimer, the regulatory subunit of the DNA-dependent protein kinase». England. Nucleic Acids Res. 29 (6): 1300–7. PMC 29753 . PMID 11238996. doi:10.1093/nar/29.6.1300 
  4. ↑ Schild-Poulter, C; Pope L, Giffin W, Kochan J C, Ngsee J K, Traykova-Andonova M, Haché R J (2001). «The binding of Ku antigen to homeodomain proteins promotes their phosphorylation by DNA-dependent protein kinase». United States. J. Biol. Chem. 276 (20): 16848–56. ISSN 0021-9258. PMID 11279128. doi:10.1074/jbc.M100768200 
  5. ↑ Goudelock, Dawn Marie; Jiang Kecheng, Pereira Elizabeth, Russell Beatriz, Sanchez Yolanda (2003). «Regulatory interactions between the checkpoint kinase Chk1 and the proteins of the DNA-dependent protein kinase complex». United States. J. Biol. Chem. 278 (32): 29940–7. ISSN 0021-9258. PMID 12756247. doi:10.1074/jbc.M301765200 
  6. ↑ Karmakar, Parimal; Snowden Carey M, Ramsden Dale A, Bohr Vilhelm A (2002). «Ku heterodimer binds to both ends of the Werner protein and functional interaction occurs at the Werner N-terminus». England. Nucleic Acids Res. 30 (16): 3583–91. PMC 134248 . PMID 12177300. doi:10.1093/nar/gkf482 
  7. ↑ Li, B; Comai L (2000). «Functional interaction between Ku and the werner syndrome protein in DNA end processing». UNITED STATES. J. Biol. Chem. 275 (37): 28349–52. ISSN 0021-9258. PMID 10880505. doi:10.1074/jbc.C000289200 
  8. ↑ Shao, R G; Cao C X, Zhang H, Kohn K W, Wold M S, Pommier Y (1999). «Replication-mediated DNA damage by camptothecin induces phosphorylation of RPA by DNA-dependent protein kinase and dissociates RPA:DNA-PK complexes». ENGLAND. EMBO J. 18 (5): 1397–406. ISSN 0261-4189. PMC 1171229 . PMID 10064605. doi:10.1093/emboj/18.5.1397 
  9. ↑ Song, K; Jung Y, Jung D, Lee I (2001). «Human Ku70 interacts with heterochromatin protein 1alpha». United States. J. Biol. Chem. 276 (11): 8321–7. ISSN 0021-9258. PMID 11112778. doi:10.1074/jbc.M008779200 
  10. ↑ Grandvaux, N; Grizot S, Vignais P V, Dagher M C (1999). «The Ku70 autoantigen interacts with p40phox in B lymphocytes». ENGLAND. J. Cell. Sci. 112 ( Pt 4): 503–13. ISSN 0021-9533. PMID 9914162 
  11. ↑ Goedecke, W; Eijpe M, Offenberg H H, van Aalderen M, Heyting C (1999). «Mre11 and Ku70 interact in somatic cells, but are differentially expressed in early meiosis». UNITED STATES. Nat. Genet. 23 (2): 194–8. ISSN 1061-4036. PMID 10508516. doi:10.1038/13821  Parâmetro desconhecido |DUPLICATE DATA: pmid= ignorado (ajuda)
  12. ↑ Ko, L; Cardona G R, Chin W W (2000). «Thyroid hormone receptor-binding protein, an LXXLL motif-containing protein, functions as a general coactivator». UNITED STATES. Proc. Natl. Acad. Sci. U.S.A. 97 (11): 6212–7. ISSN 0027-8424. PMC 18584 . PMID 10823961. doi:10.1073/pnas.97.11.6212 
  13. ↑ Ko, Lan; Chin William W (2003). «Nuclear receptor coactivator thyroid hormone receptor-binding protein (TRBP) interacts with and stimulates its associated DNA-dependent protein kinase». United States. J. Biol. Chem. 278 (13): 11471–9. ISSN 0021-9258. PMID 12519782. doi:10.1074/jbc.M209723200 
  14. ↑ Gell, D; Jackson S P (1999). «Mapping of protein-protein interactions within the DNA-dependent protein kinase complex». ENGLAND. Nucleic Acids Res. 27 (17): 3494–502. PMC 148593 . PMID 10446239. doi:10.1093/nar/27.17.3494 
  15. ↑ Yang, C R; Yeh S, Leskov K, Odegaard E, Hsu H L, Chang C, Kinsella T J, Chen D J, Boothman D A (1999). «Isolation of Ku70-binding proteins (KUBs)». ENGLAND. Nucleic Acids Res. 27 (10): 2165–74. ISSN 0305-1048. PMC 148436 . PMID 10219089. doi:10.1093/nar/27.10.2165 
  16. ↑ Singleton, B K; Torres-Arzayus M I, Rottinghaus S T, Taccioli G E, Jeggo P A (1999). «The C terminus of Ku80 activates the DNA-dependent protein kinase catalytic subunit». UNITED STATES. Mol. Cell. Biol. 19 (5): 3267–77. ISSN 0270-7306. PMC 84121 . PMID 10207052 
  17. ↑ a b Song, K; Jung D, Jung Y, Lee S G, Lee I (2000). «Interaction of human Ku70 with TRF2». NETHERLANDS. FEBS Lett. 481 (1): 81–5. ISSN 0014-5793. PMID 10984620. doi:10.1016/S0014-5793(00)01958-X 
  18. ↑ Romero, F; Dargemont C, Pozo F, Reeves W H, Camonis J, Gisselbrecht S, Fischer S (1996). «p95vav associates with the nuclear protein Ku-70». UNITED STATES. Mol. Cell. Biol. 16 (1): 37–44. ISSN 0270-7306. PMC 230976 . PMID 8524317 
  19. ↑ Chai, Weihang; Ford Lance P, Lenertz Lisa, Wright Woodring E, Shay Jerry W (2002). «Human Ku70/80 associates physically with telomerase through interaction with hTERT». United States. J. Biol. Chem. 277 (49): 47242–7. ISSN 0021-9258. PMID 12377759. doi:10.1074/jbc.M208542200 
  20. ↑ Ohta, Satoshi; Shiomi Yasushi, Sugimoto Katsunori, Obuse Chikashi, Tsurimoto Toshiki (2002). «A proteomics approach to identify proliferating cell nuclear antigen (PCNA)-binding proteins in human cell lysates. Identification of the human CHL12/RFCs2-5 complex as a novel PCNA-binding protein». United States. J. Biol. Chem. 277 (43): 40362–7. ISSN 0021-9258. PMID 12171929. doi:10.1074/jbc.M206194200 
  21. ↑ Balajee, A S; Geard C R (2001). «Chromatin-bound PCNA complex formation triggered by DNA damage occurs independent of the ATM gene product in human cells». England. Nucleic Acids Res. 29 (6): 1341–51. PMC 29758 . PMID 11239001. doi:10.1093/nar/29.6.1341 
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